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Membrane Protein Purification and Crystallization - A Practical Guide (Cód: 3027514)

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Membrane Protein Purification and Crystallization - A Practical Guide

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Descrição

This second edition ofMembrane Protein Purification and Crystallization, A Practical Guide is written for bench scientists working in the fields of biochemistry, biology, and proteomic research. This guide presents isolation and crystallization techniques in a concise form, emphasizing the critical aspects unique to membrane proteins. It explains the principles of the methods and provides protocols of general use, permitting researchers and students new to this area to adapt these techniques to their particular needs. This edition is not only an update but is comprised mainly of new contributions. It is the first monograph compiling the essential approaches for membrane protein crystallization, and emphasizes recent progress in production and purification of recombinant membrane proteins.Provides general guidelines and strategies for isolation and crystallization of membrane proteinsGives detailed protocols that have wide application, and low specialized equipment needsEmphasizes recent progress in production and purification of recombinant membrane proteins, especially of histidine-tagged and other affinity-epitope-tagged proteins Summarizes recent developments of Blue-Native PAGE, a high resolution separation technique, which is independent of the use of recombinant techniques, and is especially suited for proteomic analyses of membrane protein complexesGives detailed protocols for membrane protein crystallization, and describes the production and use of antibody fragments for high resolution crystallizationPresents a comprehensive guide to 2D-crystallization of membrane proteins

Características

Peso 0.00 Kg
Produto sob encomenda Sim
Marca ELSEVIER S&T
Acabamento e-book
Territorialidade Internacional
Proteção Drm Sim
Tamanho do Arquivo 18713
Início da Venda 05/01/2003
Cód. Barras 9780080536170
Ano da edição 12009